Affinity chromatography of bacterial lactate dehydrogenases
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چکیده
منابع مشابه
Affinity Chromatography of Potato Lactate Dehydrogenase
NH,CI. None of the adaptations could be attributed to the high-protein diet per se since they were not evident in those rats fed a high-protein diet and given NaHC03 solution to drink. Of particular interest is the increased rate of gluconeogenesis found in kidney slicesfrom rats fed ona high-protein diet. Krebs et al. (1963) have observed an increased rate of renal gluconeogenesis in rats fed ...
متن کاملStudies on the active center of D- and L-lactate dehydrogenases using oxamate-diaminohexyl-Sepharose affinity chromatography.
Vertebrate and invertebrate L-lactate dehydrogenases (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) are effectively bound to oxamate-diaminohexyl-Sepharose, whereas several D-lactate dehydrogenases (D-lactate:NAD+ oxidoreductase, EC 1.1.1.28) do not bind to the same Sepharose. One explanation for our findings is that the enzymes' substrate is oriented in a reversed manner in the active center of ...
متن کاملAffinity chromatography of lactate dehydrogenase on immobilized nucleotides.
The interaction of two isoenzymes of lactate dehydrogenase from pig heart muscle (H(4)) and rabbit skeletal muscle (M(4)), with immobilized nucleotides was examined: the effects of pH and temperature on the binding of lactate dehydrogenase were studied with immobilized NAD(+) matrices. The influence of substrate, product and sulphite on the binding of heart muscle lactate dehydrogenase to immob...
متن کاملIsolation of human lactate dehydrogenase isoenzyme X by affinity chromatography.
Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the othe...
متن کاملPreparation of Plasminogen by Affinity Chromatography
Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1710543